Sequencing of a 1,3-1,4-b-D-Glucanase (Lichenase) from the Anaerobic Fungus Orpinomyces Strain PC-2: Properties of the Enzyme Expressed in Escherichia coli and Evidence that the Gene Has a Bacterial Origin

A 971-bp cDNA, designated licA, was obtained from a library of Orpinomyces sp. strain PC-2 constructed in Escherichia coli. It had an open reading frame of 738 nucleotides encoding LicA (1,3-1,4-b-D-glucanase; lichenase) (EC 3.2.1.73) of 245 amino acids with a calculated molecular mass of 27,929 Da. The deduced amino acid sequence had high homology with bacterial b-glucanases, particularly in the central regions and toward the C-terminal halves of bacterial enzymes. LicA had no homology with plant b-glucanases. The genomic DNA region coding for LicA was devoid of introns. More than 95% of the recombinant b-glucanase produced in E. coli cells was found in the culture medium and periplasmic space. A N-terminal signal peptide of 29 amino residues was cleaved from the enzyme secreted from Orpinomyces, whereas 21 amino acid residues of the signal peptide were removed when the enzyme was produced by E. coli. The b-glucanase produced by E. coli was purified from the culture medium. It had a molecular mass of 27 kDa on sodium dodecyl sulfate-polyacrylamide gels. The Km and Vmax values with lichenin as the substrate at pH 6.0 and 40°C were 0.75 mg/ml and 3,790 mmol/min/mg, respectively. With barley b-glucan as the substrate, the corresponding values were 0.91 mg/ml and 5,320 mmol/min/mg. This enzyme did not hydrolyze laminarin, carboxymethylcellulose, pustulan, or xylan. The main products of lichenin and barley b-glucan hydrolysis were triose and tetraose. LicA represented the first 1,3-1,4-b-D-glucanase reported from fungi. The results presented suggest that licA of Orpinomyces had a bacterial origin.